Contains:• 100 µl Taq DNA Polymerase (5 U/µl)• 2.5 ml 10X PCR buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)• 1 ml Magnesium Chloride (50 mM)Store at -30°C to -10°C in a non-frost-free freezer. Guaranteed stable for 6 months when stored properly.
描述
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.
With Life Technologies' Taq DNA Polymerase, you get:• Your choice of recombinant or native enzyme
• Amplification of PCR products up to 5 kb in size
• An enzyme that is licensed and qualified for PCR
Applications
Taq DNA Polymerase is appropriate for use in the amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing.
Source
Recombinant enzyme is purified from the cloned
Thermus aquaticus DNA polymerase gene expressed in
E. coli.
Unit definition
One unit of
Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.